FastQCFastQC Report
Sun 5 Jun 2022
EGAF00004675350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51550876
Sequences flagged as poor quality0
Sequence length75
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACCACACCCAAGATC1630740.3163360405359552No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACTACACCCAAGATC1463470.28388848329172917No Hit
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA665430.1290821905722805Illumina Single End PCR Primer 1 (100% over 21bp)
CAAACTACAAACTACAATAACACAATCTCAACTCACTACAAACTCCACTTCCCAAGATCGGAAGAGCGTCGTGTA634410.12306483404860086Illumina Single End PCR Primer 1 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)