FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004675351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65235719
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2850380.4369354770198823TruSeq Adapter, Index 1 (97% over 44bp)
TGGGTGTGGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC1017800.15601882152935265No Hit
TGGGTGTAGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC964350.14782545740010927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)