FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004675353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57273549
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3476670.6070289096280728TruSeq Adapter, Index 2 (97% over 44bp)
TGGGTGTGGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC1136710.1984703270265302No Hit
TGGGTGTAGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC1091740.1906185349191474No Hit
TGGGTGTGGTGGTTTATGTTTGTAATTTTAGTATTTTGGGAGGTTGAGGTGGGTGGATTATGAGGTTAGGAGATT654880.11434248644169055No Hit
TGGGATGGTTTTGATTTTTTGATTTTGTGATTTGTTTGTTTTGGTTTTTTAAAGTGTTGGGATTATAGGTGTGAG592880.10351724493273501No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)