FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004675355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57417003
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4992070.8694410608648453TruSeq Adapter, Index 3 (97% over 44bp)
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT655970.11424664571921318Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTGGGATTATAGGCGTGAG654050.11391225000023075No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC594480.10353727448992767No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)