FastQCFastQC Report
Thu 12 May 2022
EGAF00004675357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52939643
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2125930.4015761874329224TruSeq Adapter, Index 4 (97% over 44bp)
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTGGGATTATAGGCGTGAG656900.12408470529353588No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT573720.10837247240220338Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC552050.10427913161409116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)