FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004675361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59998081
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7759431.293279696728967TruSeq Adapter, Index 6 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA970560.16176517378947505TruSeq Adapter, Index 6 (97% over 41bp)
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT800600.1334376011126089Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT746410.12440564557389759Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTGGGATTATAGGCGTGAG718900.11982049892562396No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)