FastQCFastQC Report
Wed 11 May 2022
EGAF00004675367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58739195
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA21337853.632642565156026TruSeq Adapter, Index 27 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1791790.3050416336144886TruSeq Adapter, Index 27 (97% over 44bp)
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT1233370.20997393648312682Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC1057510.18003481321117865No Hit
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT1044320.17778929384374437Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA849750.1446649039027518TruSeq Adapter, Index 27 (97% over 41bp)
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTGGGATTATAGGCGTGAG816330.13897534687017757No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCGGGCGGATTACGAGGTTAGGAGATC632510.10768108075025543No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA632260.10763851973116076TruSeq Adapter, Index 27 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)