FastQCFastQC Report
Thu 12 May 2022
EGAF00004675371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66892182
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA16952952.534369412557061TruSeq Adapter, Index 5 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2490000.372240809845312TruSeq Adapter, Index 5 (97% over 44bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC1231650.184124656002401No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT1132700.16933219490433127Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT966480.14448325216839242Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGTGTGGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC771270.1153004696423268No Hit
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTGGGATTATAGGCGTGAG717990.107335413277444No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC698070.10435748679868151No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)