FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004675372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66892182
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACGCCCGCCACTACGCCCGAGATC1149450.1718362244484714No Hit
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA1069890.15994245784955854Illumina Single End PCR Primer 1 (100% over 21bp)
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAATCCGAGATCGGAAGAGCGTCGTGTA922060.1378427153116339Illumina Single End PCR Primer 1 (100% over 21bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA777320.11620491016424012Illumina Single End PCR Primer 1 (100% over 57bp)
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACGAACGAATCACGAAATCAAAAAATC742400.1109845691683372No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACCACACCCAAGATC729510.10905758762660785No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)