FastQCFastQC Report
Thu 12 May 2022
EGAF00004675374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56237707
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACCACACCCAAGATC966680.17189178783551753No Hit
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA896970.15949618998512866Illumina Single End PCR Primer 1 (100% over 21bp)
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACTACACCCAAGATC873310.1552890483248188No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACGCCCGCCACTACGCCCGAGATC862530.15337218496479593No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA843900.15005946099473794Illumina Single End PCR Primer 1 (100% over 57bp)
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAATCCGAGATCGGAAGAGCGTCGTGTA733600.1304462857989569Illumina Single End PCR Primer 1 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)