FastQCFastQC Report
Sun 5 Jun 2022
EGAF00004675375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58496231
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA19137493.271576590977289TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2773100.4740647307687225TruSeq Adapter, Index 13 (97% over 40bp)
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT1248910.21350264429857027Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT1086730.18577778113601884Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC1078450.18436230532527811No Hit
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTGGGATTATAGGCGTGAG695030.11881620202163111No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC601020.1027450811318083No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)