FastQCFastQC Report
Thu 12 May 2022
EGAF00004675376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58496231
Sequences flagged as poor quality0
Sequence length75
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA1187240.20296008472751007Illumina Single End PCR Primer 1 (100% over 21bp)
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAATCCGAGATCGGAAGAGCGTCGTGTA1044430.1785465460159305Illumina Single End PCR Primer 1 (100% over 21bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACGCCCGCCACTACGCCCGAGATC1006070.1719888585642381No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA859150.14687271048283435Illumina Single End PCR Primer 1 (100% over 57bp)
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACGAACGAATCACGAAATCAAAAAATC706410.12076162650547519No Hit
CAAAATAATCTCGATCTCCTAACCTCGTAATCCGCCCGCCTCGACCTCCCAAAATACTAAAATTACAAACGTAAA602160.10293996548256246No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)