FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004675378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60726394
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA1378460.2269952008018128Illumina Single End PCR Primer 1 (100% over 21bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACGCCCGCCACTACGCCCGAGATC1332510.21942847454436368No Hit
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAATCCGAGATCGGAAGAGCGTCGTGTA1211800.19955079170352186Illumina Single End PCR Primer 1 (100% over 21bp)
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACGAACGAATCACGAAATCAAAAAATC912900.1503300195957626No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAAACGCCCGCCACCGCGCCCGAGATC779540.12836922277980148No Hit
CAAAATAATCTCGATCTCCTAACCTCGTAATCCGCCCGCCTCGACCTCCCAAAATACTAAAATTACAAACGTAAA777470.12802834958387288No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA742630.1222911408176155Illumina Single End PCR Primer 1 (100% over 57bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)