FastQCFastQC Report
Wed 11 May 2022
EGAF00004675383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50666404
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1388660.2740790524624562TruSeq Adapter, Index 9 (97% over 44bp)
TGGGTGTGGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC983390.19409113778826698No Hit
TGGGTGTAGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC884880.17464827383447226No Hit
TGGGATGGTTTTGATTTTTTGATTTTGTGATTTGTTTGTTTTGGTTTTTTAAAGTGTTGGGATTATAGGTGTGAG620670.12250129296722934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)