FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004675390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51108107
Sequences flagged as poor quality0
Sequence length20-75
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACGCCCGCCACTACGCCCG766840.1500427319681396No Hit
AACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCG569950.11151851114344735No Hit
AAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAATCCG521280.10199556011730194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTATG306.134386E-5708.282969
TATAGGT306.134386E-5708.282969
TGATTTG209.4634604E-5612.9233468
GATCGTC16800.0474.285968
TGGGATC502.8359875E-4424.9697369
AGTCGTT303.1900135E-4408.6155468
ATCGTCG37350.0398.2313869
TTAGGTA355.062526E-4350.2418868
CCTTGTT656.224073E-4326.899869
TCTGGTG656.224073E-4326.899869
TCGGGGT707.7709754E-4303.549869
AGTTGCG350.0035152389303.549869
CAGCAGG759.554586E-4283.313269
GGTGATG759.554586E-4283.313269
AGTGGTG800.001159164265.606169
AGGGCAG500.0014732495245.1693468
GGGGATC900.001649286236.0943169
CGCCAGG1401.8189894E-12227.6623569
GTGAGTC600.0025426636204.3077768
TGAGCAA600.0025426636204.3077768
Produced by FastQC (version 0.11.5)