FastQCFastQC Report
Thu 12 May 2022
EGAF00004675394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51773512
Sequences flagged as poor quality0
Sequence length75
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACCACACCCAAGATC1283000.24781011572095013No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACTACACCCAAGATC1202100.23218436485436802No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA873460.16870789062947864Illumina Single End PCR Primer 1 (100% over 57bp)
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA560080.10817886953467634Illumina Single End PCR Primer 1 (100% over 21bp)
CAAACTACAAACTACAATAACACAATCTCAACTCACTACAAACTCCACTTCCCAAGATCGGAAGAGCGTCGTGTA520430.10052051326941082Illumina Single End PCR Primer 1 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)