FastQCFastQC Report
Thu 12 May 2022
EGAF00004675395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60212170
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA17391162.8883131101237507TruSeq Adapter, Index 2 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1858400.30864192404957336TruSeq Adapter, Index 2 (97% over 44bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC824870.13699390007036782No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT778960.12936919562938853Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGTGTGGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC773330.1284341687070903No Hit
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT709100.11776688998254008Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGTGTAGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC654190.10864747110094189No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)