FastQCFastQC Report
Thu 12 May 2022
EGAF00004675398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67197181
Sequences flagged as poor quality0
Sequence length75
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACGCCCGCCACTACGCCCGAGATC1168960.1739596784573448No Hit
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA962120.14317862530572525Illumina Single End PCR Primer 1 (100% over 21bp)
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAATCCGAGATCGGAAGAGCGTCGTGTA840310.12505137678320166Illumina Single End PCR Primer 1 (100% over 21bp)
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACGAACGAATCACGAAATCAAAAAATC705190.10494339040799941No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA685220.10197153955014869Illumina Single End PCR Primer 1 (100% over 57bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAAACGCCCGCCACCGCGCCCGAGATC673830.10027652796923132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)