FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004675401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49150116
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3006780.611754405625411TruSeq Adapter, Index 9 (97% over 44bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC776640.15801386918395066No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA713150.14509630048482489TruSeq Adapter, Index 12 (97% over 44bp)
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT633230.12883591159784852Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTGGGATTATAGGCGTGAG594700.12099666255111177No Hit
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT544960.11087664574382693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)