FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004675403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51116901
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4087700.7996768035683541TruSeq Adapter, Index 10 (97% over 44bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC1363100.2666632705296434No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT1008490.19729091166931267Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTGGGATTATAGGCGTGAG955210.18686774458412495No Hit
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT862180.168668284487747Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC803850.1572571858376156No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCGGGCGGATTACGAGGTTAGGAGATC718370.1405347323383317No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTGAGATC709680.13883470752657717No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTGAGATC633150.1238631426423914No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGTTATCGCGTTTGAGATC571080.11172038774416312No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)