FastQCFastQC Report
Sun 5 Jun 2022
EGAF00004675404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51116901
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACGCCCGCCACTACGCCCGAGATC1350880.2642726717724926No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACGAACGAATCACGAAATCAAAAAATC1241060.24278858376019313No Hit
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA988140.193309840907609Illumina Single End PCR Primer 1 (100% over 21bp)
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAATCCGAGATCGGAAGAGCGTCGTGTA847870.16586881900371855Illumina Single End PCR Primer 1 (100% over 21bp)
CAAAATAATCTCGATCTCCTAACCTCGTAATCCGCCCGCCTCGACCTCCCAAAATACTAAAATTACAAACGTAAA840790.16448375851266883No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAAACGCCCGCCACCGCGCCCGAGATC792730.15508178009461096No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACAAAAAAATAACGTAAACCCGAGATC702470.13742421513385564No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAACAAAAAAATAACGTAAACCCGAGATC628820.12301606468670705No Hit
CAAACGCGATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAACAAAAAAATAACGTAAACCCGAGATC568860.11128608911561363No Hit

[FAIL]Adapter Content

Adapter graph