FastQCFastQC Report
Thu 12 May 2022
EGAF00004675442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59033111
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA17657832.991173885448795TruSeq Adapter, Index 12 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2177370.3688387691443197TruSeq Adapter, Index 12 (97% over 44bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC854610.1447679083014954No Hit
TGGGTGTGGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC777350.13168033783616792No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT679730.11514385545427208Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGTGTAGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC662200.1121743355182484No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA630980.10688577805089756TruSeq Adapter, Index 12 (97% over 41bp)
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT615160.10420592606071531Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)