FastQCFastQC Report
Thu 14 Sep 2023
EGAF00004675742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55269445
Sequences flagged as poor quality0
Sequence length98
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2558970.4629990404282149No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA656900.11885409741313668No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG624550.11300095378196759Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGG621360.1124237813497132No Hit
GTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAA588940.10655797249275799No Hit
GCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTG560170.10135256469465181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)