FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004677551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004677551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12008984
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG391440.32595596763223267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA186030.15490902477678378No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT156000.12990274614405348No Hit
CTCGGTTTTATTCTTATTGCTCAGGCTTGAGTGCACTGGCCTCTTCTCAGC146750.12220017946564005No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG128060.10663683122568904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT78100.027.4148271
CCGGCTT92850.024.5212652
TTCACGC103700.022.4508887
TCACGCT107650.021.6897838
CGGCTTC110250.021.546343
CTCGGTT44450.021.538431
GCGGACT20250.021.4425542
GGCGGAC23650.021.0541131
GTCCCGT12150.021.0497821
CACGCTC113700.020.49619
ACGCTCA118800.019.67302110
AGTGCAC40550.019.47748630
AACGCTA120600.019.27093123
ACGCTAC121250.019.18713824
TCGGTTT52100.019.1729742
CGCTACC121800.019.00916325
GTCCGTT12600.018.6813491
AAAACGC124300.018.66016821
CCCCCCT6950.018.5624581
GAAAACG124700.018.43663420
Produced by FastQC (version 0.11.5)