FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004679169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56629504
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC41350.014.67226446
TCGTATG42300.014.17906645
TATGCCG44650.012.96655548
CTCGTAT49800.011.20853344
ACACGTC79850.08.64150713
TCTCGTA68350.08.521605543
GATCGGA78950.08.1942151
CGTCTGA84800.07.890861516
ATGCCGT82100.07.558480349
ACGTCTG92500.07.38404615
ATCTCGT79450.07.243511742
ATCGGAA96300.06.77907132
AGATCGG91050.06.588385670
GATCTCG90450.06.362699541
GCCGTCT107500.06.22400651
CACGTCT110350.06.221475614
GCACACG119700.06.0250811
TGCCGTC109050.05.97666250
CACACGT124100.05.86775312
GTATGCC119950.05.66488147
Produced by FastQC (version 0.11.5)