FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004679177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54737822
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTCG43100.019.58094835
TCGTATG45350.018.29316945
CGTATGC44050.018.20530146
TATGCCG47700.017.10209848
CTCGTAT52500.013.82673844
ACTCGAT62850.012.48693737
AACTCGA71850.011.41649736
ACACGTC81050.010.74704413
GATCGGA83700.010.3998871
ATGCCGT83350.010.0770649
TCTCGTA68650.010.02010543
CGTCTGA86000.09.92620616
ATCGGAA98200.09.3047972
ACGTCTG95200.09.03984615
ATCTCGT79400.08.79368342
ACCTAAC106750.08.6075232
CACGTCT110450.07.917396514
GCCGTCT109050.07.892287351
CTCGATC91100.07.854957638
TGCCGTC112900.07.80697850
Produced by FastQC (version 0.11.5)