FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004679219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71575275
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC55750.015.31429246
TCGTATG56700.014.93680145
TATGCCG57900.014.32358948
CTCGTAT65400.012.84583544
TATCTCG63350.012.38797341
GATCGGA90500.09.7227911
TCTCGTA89500.09.38707143
ACACGTC101400.09.23563113
CGTCTGA109000.08.59041216
ATGCCGT105950.08.51178749
ATCTCGT102100.08.3298642
ATCGGAA113100.08.1686682
ACGTCTG120250.07.95948115
CACGTCT139150.07.077490314
TGCCGTC138900.06.791204550
GCCGTCT137100.06.62829751
GCACACG155200.06.234494711
GTATGCC155200.06.19094947
CACACGT160650.06.17361412
GACACTA162650.06.149874736
Produced by FastQC (version 0.11.5)