FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004679226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15988857
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT21750.098.0016256
ACGACGC22100.097.429785
CACGACG24650.087.646044
GACGCTC33150.064.7369167
TACACGA38150.057.406112
ACGCTCT39800.053.737828
CTACACG41750.052.7711221
ACACGAC43700.049.768413
CGCTCTT54300.039.78859
CGCTTAG14450.00282977086.0988081
CGCATAG14700.00342777375.99508671
TAACCCT500100.05.9729046
CGATGGA27202.2083623E-75.93998431
CGCTGAG34203.086825E-95.79787971
CTAACCC522800.05.7826655
CCTAACC539950.05.67962654
CCCTAAC539650.05.6153663
TCTTCCG91350.05.60340410-14
ACCCTAA548250.05.568642
AACCCTA543450.05.51356031
Produced by FastQC (version 0.11.5)