FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004679229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19369045
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12450.085.580579
ACGACCG28200.081.240221
CGCCGAA28950.079.901381
CGGTACG33000.077.709491
TGCGCGA30350.075.2416461
TTTGCGC33850.074.666531
GTGCGAC31150.073.5465241
GCGCTAG33550.072.250331
CGAACGC35900.067.314991
GCGCGTA32600.065.7413941
CCGCTAT38550.065.563211
ATAACGC34850.065.526081
GACGCGT32750.064.763321
AACCGCG37800.064.322451
CGAACTA40300.063.9998361
AACGCGT27200.063.491463
TCGCGAT23250.063.2849278
CGCGGTA40800.063.215531
GTACCGT46000.063.0273938
CTTACCG44150.061.7666631
Produced by FastQC (version 0.11.5)