FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004679234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18453518
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT24950.092.1039666
ACGACGC26050.088.49475
CACGACG27650.083.373394
GACGCTC36250.063.1927877
TACACGA38650.061.147532
CTACACG44200.053.825161
ACGCTCT44550.051.7449348
ACACGAC48100.048.981693
CGCTCTT57900.040.3150259
GTTCCGA51000.06.3964675
TAACCCT718450.06.1448376
ATCGGGT18301.6784765E-46.01870061
CTAACCC760950.05.97323045
AACCCTA773150.05.9167991
ACCCTAA786850.05.8320682
CCCTAAC792500.05.76284273
CCTAACC789000.05.75165464
CGAAAAG31701.2787132E-75.5592291
CGCTGAG35758.756615E-95.54562231
CGCCAAT15950.0084854215.52437451
Produced by FastQC (version 0.11.5)