FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004679237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19593186
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG29700.081.550411
ACGACCG31050.081.0871
TGCGCGA31100.080.48321
CGCCGAA29550.080.220451
TTACGCG11300.078.262029
TTTGCGC31500.077.124091
GCGCTAG36700.074.2202451
GTGCGAC33800.072.529431
GACGCGT32250.068.9388351
ATAACGC36400.068.562251
CGAACGC37850.067.102691
AACCGCG37000.066.853521
TAGGCGC37250.066.404831
CGCGGTA36350.064.606021
GTACCGT42300.064.43438
GCGCGTA33800.064.034991
CCGCTAT36550.063.8496631
CGAACTA39450.063.44811
CCGATAA39650.063.313731
TTACCCG36150.062.926981
Produced by FastQC (version 0.11.5)