FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004679238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19593186
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT25850.0100.1179356
ACGACGC27650.093.861775
CACGACG29650.087.041694
GACGCTC39550.065.437387
TACACGA43400.061.1790472
CTACACG47600.056.292221
ACGCTCT47350.054.659378
ACACGAC51650.050.9533353
CGCTCTT62750.041.1294259
AACGGGG32900.07.6074131
GTTCCGA58200.06.2279895
TAACCCT890800.06.16055546
CTAACCC954950.05.95163545
AACCCTA953150.05.7151081
CCTAACC988850.05.6816344
CCCTAAC998550.05.67771053
ACCCTAA983100.05.63038642
CGATGAG27852.0461866E-65.5507041
TCGCCGG19655.2272325E-45.535616145
ATTGGGG128250.05.2806141
Produced by FastQC (version 0.11.5)