FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004679239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17089579
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11150.079.3072749
TTTGCGC30400.078.8102951
CGCCGAA27850.078.060921
TGCGCGA26450.077.719611
ACGACCG25300.074.822281
GTGCGAC28850.071.510511
CGGTACG28450.071.476291
CGAACGC32750.069.768391
GCGCGTA26350.067.0700761
TCGTACG34450.066.906148
ATAACGC34650.066.796331
GTTCGCG37250.065.9631658
CCGCTAT34400.064.917241
CGAACGT17900.064.450463
GACGCGT26200.064.0672451
AACGCGT24950.063.97853
GCCGGAT34950.063.8956451
TAGGCGC32500.063.4793361
GGCGACT35450.062.994441
TCCGCGA25100.062.3746458
Produced by FastQC (version 0.11.5)