FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004679240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17089579
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT22300.090.7146
ACGACGC23250.088.24945
CACGACG26250.078.44274
GACGCTC34850.057.630447
TACACGA37600.054.9693952
CTACACG39250.053.342531
ACACGAC41900.049.487033
ACGCTCT42150.047.476938
CGCTCTT56300.036.8322949
CGGGTTA25506.200207E-86.3379811
CGAAAAG32307.930794E-106.14086681
CTCGCGT13000.0072762346.13508379
CGATGAG25452.55347E-65.77312041
TAACCCT420350.05.67492066
CTAACCC432250.05.50157645
ACCCTAA467750.05.3644632
CCCTAAC454950.05.32257943
AACCCTA453600.05.3121361
CCTAACC449800.05.30322364
TCTTCCG98150.05.259787610-14
Produced by FastQC (version 0.11.5)