FastQCFastQC Report
Tue 13 Aug 2024
EGAF00004679324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6951647
Total Bases451.8 Mbp
Sequences flagged as poor quality0
Sequence length65
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTAT4364056.277720948719059TruSeq Adapter, Index 16 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTATG529680.7619489309511832TruSeq Adapter, Index 16 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)