FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004679966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8831891
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA288760.3269514988353004No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN271520.3074313303911925No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG198410.22465177615982804No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA197800.22396109734597042No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG123620.13997002453947857No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG108160.12246527951941436No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT102400.11594345990003727No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA92750.10501714751687946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG4850.016.69951831
CGCGTAA850.002559978315.8888623
AACTCTA28400.015.1313645
CATGCAT16100.014.6765899
GTTGTCG6350.014.525526
TATATAC13550.013.4534787
ACGCTCG2855.4569682E-1113.41968518
GTCACTC55300.013.3887458
TACGCTA6400.013.35778623
GGAGGTC55750.013.2833924
GGGAGGT55950.013.2359833
GAGGTCA56750.012.930265
GGTCACT56050.012.888977
TTGCGTT6500.012.806313533
AGGTCAC57850.012.7212776
AGCTTAG37650.012.54923545
AGGGATC42800.012.4116962
TACATGC20950.012.3538597
AATACGC6750.012.33158621
TCACTCT62550.012.2684239
Produced by FastQC (version 0.11.5)