FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004679967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51027930
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1220460.2391748989230016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC265700.025.4709174
GACCGCG272250.024.7742165
CAGACCG280400.024.3694153
ACCGCGT279950.024.060686
CCGCGTT305000.022.091587
TCAGACC324150.021.4620362
GAACGAT310650.021.31979845
CGCGTTC319150.021.1397978
GCGTTCT348950.019.3663889
CTCAGAC398050.018.9840871
AGAACGA354850.018.5756744
ATACGGA373800.017.51722133
TACGGAG376750.017.44534534
AATACGG376700.017.38891832
TTCGACG19100.017.3149246
AAGAACG384350.017.08034743
CAATACG372600.016.6808231
CGTTCTC411400.016.65622910
ACGGAGA401650.016.39693335
GTCGGTT37700.015.5931531
Produced by FastQC (version 0.11.5)