FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004679971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48151579
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1154140.23968892068939213No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG728450.1512826817164189No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA540770.11230576675377563No Hit
CTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGG528340.10972433531203618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG23400.021.8105056
ACGCTAC239600.020.82608424
AACGCTA241550.020.70790723
CGCTACC248350.020.07417125
AGACCGC89050.019.6174014
AAAACGC260500.019.25312421
GACCGCG95150.018.454245
GAAAACG271750.018.44391420
AAACGCT272800.018.40472822
GTTTTCG296150.016.86454645
CGTGGTT301700.016.74822441
GCACTAC85600.016.72680525
TCGTGGT299250.016.7245940
CGGTATA42050.016.49233620
ACCGCGT106100.016.4861766
TCGACGA33900.016.1161697
GCTACCT317250.015.98406826
CCGCGTT174450.015.943191
CAGACCG113850.015.8773263
CTACCTC328100.015.52424127
Produced by FastQC (version 0.11.5)