FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004679999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004679999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45303254
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1754410.3872591580286926No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA507570.11203830965431313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC403050.022.630374
CAGACCG404100.022.6165563
GACCGCG407500.022.4045585
ACCGCGT416900.021.9155856
TCAGACC445850.020.6856842
CCGCGTT467000.019.5885587
CGCGTTC480350.019.0909868
CTCAGAC509200.019.002671
GAACGAT483650.018.30965445
GCGTTCT525950.017.5513279
AGAACGA558000.016.08773644
CGTTCTC590100.015.76150810
AATACGG573100.015.71261532
ATACGGA578700.015.56834333
TACGGAG579250.015.541923534
CAATACG587450.015.39007831
CCCAATA608600.014.90317629
ACGGAGA610100.014.81504935
AAGAACG609350.014.78379443
CACTCCC629800.014.6238124
Produced by FastQC (version 0.11.5)