FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004680013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004680013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35232414
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT85050.017.97761745
AGACCGC90850.016.658424
CAGACCG100950.015.3704473
ACGTTCG9300.014.9901596
TTCGACG11700.014.6057976
AGAACGA108400.014.18810444
ACCGCGT110050.013.8732196
CTACACG36400.013.71352745
GTCCGTT23000.013.7010761
GACCGCG112200.013.5285815
CGTACGC49700.013.4834535
CGCGTGT10550.013.2279851
CTCGGTT57750.013.2130221
CCGTACG51450.013.0686154
CGCGTTC119050.012.8810868
CCGCGTT118400.012.8758387
ATACGGA119900.012.85041933
TACGGAG120250.012.71861234
GTTAGTT78450.012.6972144
AATACGG120800.012.64290332
Produced by FastQC (version 0.11.5)