FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004680014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004680014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35232414
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN654740.1858345556452646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG66050.013.78202345
GGCATAG78500.011.42955535
ATAGGGG83350.011.06734838
GAGCTTA91350.010.30954244
CATAGGG91450.09.98696337
ATAGCTC45850.09.96406239
ACCCGGC50450.09.8180985
GCATAGG93900.09.67628436
TGGCATA96250.09.41410534
AGTTCAC114950.09.3623495
GGGAGGT158900.09.3236873
TCAGTTC116450.09.3195133
GTTCACC114300.09.0372876
CTCAGTT121700.08.8808072
TAGCTCT54250.08.75519340
CGGACAC67850.08.59041513
TCACCCG64750.08.5890053
ACGGACA57100.08.551702518
TGATAGC57800.08.48437337
CACCGAA121450.08.46002845
Produced by FastQC (version 0.11.5)