FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004680016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004680016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28161170
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT300750.10679598894506159No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG298930.10614970897871076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTC132650.013.2127298
AGCTTAG98550.013.19655545
GGCATAG98600.012.93951135
GGTCACT136600.012.8966387
TAGCGTA5300.012.31184337
AGGTCAC145400.012.3018296
ATAGGGG108750.012.26950838
ACCCGGC45700.011.8649825
ACTCTCC148000.011.781527511
GGGAGGT173500.011.5278743
GAGCTTA113900.011.51532944
CATAGGG113800.011.38888437
GAGGTCA164000.011.2084215
TGGCATA114650.011.18720934
GGAGGTC164400.011.1395014
GTTGTCG14750.011.13699526
ATAGCTC48200.010.78357539
CGTGGCG93550.010.7019761
GCATAGG121250.010.65209236
TCACTCT171250.010.4185139
Produced by FastQC (version 0.11.5)