FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004680019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004680019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37888475
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC93450.025.6440854
CAGACCG97050.024.9942863
GACCGCG133200.018.0081845
CTCAGAC172850.017.5682541
ACCGCGT149650.016.1187576
TCAGACC172750.015.5533872
CGCGTAA11500.014.6807931
CCGTACG41800.013.8348284
CGTACGC43200.013.5948285
CCGGTAT29200.013.0283641
TTCGACG10400.012.9816956
CCGCGTT185750.012.9720217
GCCTACG19250.012.73921810
ATTTCGT60050.012.7382437
CTCGGTT53250.012.5551781
CGCGTTT32000.012.5214921
CGGCCTA21500.011.9292578
GCGTTCT171700.011.910799
TCCGTAC49800.011.8834873
GTACGCC52500.011.5722546
Produced by FastQC (version 0.11.5)