FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004680036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004680036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39829200
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG139400.014.40151638
AGCTTAG141600.014.235723545
GGCATAG149150.013.45977935
CATAGGG167650.012.189054537
TGGCATA166600.012.17148134
GGAGGTC214700.012.0891064
GAGCTTA168550.012.02501344
GGGAGGT217350.012.0244733
GCATAGG180900.011.35861836
ACTCTCC226900.010.99893411
GAGGTCA244400.010.8498255
GTCACTC233850.010.7857568
GGTCACT234100.010.6106857
GTTGTCG21350.010.43698826
AGGTCAC261600.09.8225916
AGGGATC107000.09.6518282
TCACTCT273600.09.4080019
CACTCTC268250.09.2615710
TCTCCCC282850.08.96375313
TGGGAGG240350.08.9400482
Produced by FastQC (version 0.11.5)