FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004680040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004680040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40949218
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1243560.30368345495633153No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG680370.16614969301733676No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG675560.16497506741154372No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT555080.13555326013795918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACT215400.012.7356657
AGGTCAC221500.012.6003526
GTCACTC219400.012.4716228
AGCTTAG158500.012.06481645
ATAGGGG156600.012.05291838
TTTCTAT587800.011.9425831
GGGAGGT252850.011.8629883
TTCTATA591000.011.8314972
GGAGGTC242250.011.7486174
GGCATAG161050.011.71989835
TCTATAG589450.011.6253983
GTTGTCG22900.011.59227226
AGGGATC177050.011.5365762
GAGGTCA248800.011.4741035
ACTCTCC246050.011.20106111
TCACTCT264100.010.6750519
TGGCATA179200.010.67095934
CTATAGT643850.010.627954
CACTCTC258100.010.60006310
AGACTCA208550.010.5808214
Produced by FastQC (version 0.11.5)