FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004680047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004680047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9645094
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT522600.5418298670806111No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA124960.1295580945089804No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG100970.10468534573120801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG102350.025.0600725
AGACCGC101500.024.8490284
ACCGCGT104850.024.6127576
CAGACCG105050.023.8165343
TCAGACC111150.022.7726142
CCGCGTT114900.022.4595977
CGCGTTC117950.021.8595268
GAACGAT115450.021.73180445
CTCAGAC123850.020.9984321
GCGTTCT123850.020.8726739
AGAACGA131000.019.2554544
AATACGG134750.018.96928232
TACGGAG135250.018.88271534
ATACGGA136150.018.77432333
CAATACG137700.018.65910131
AAGAACG136900.018.45856343
CCCAATA142150.018.10672829
CGTTCTC144750.017.99882110
CTACACG10400.017.95806745
ACGGAGA143800.017.77573435
Produced by FastQC (version 0.11.5)