FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004680048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004680048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9645094
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT140660.14583580004507993No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA111180.1152710383123275No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG109430.11345664438314443No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG96610.10016491285621477No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTC96500.100050865237809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG17200.013.08930345
TGCCCGT38450.012.47170844
GGCATAG18550.012.25578735
CGCTAAT2056.4073945E-510.97597233
GAGCTTA21150.010.96401344
GCCCGTG46100.010.64634545
CATAGGG22100.010.39035237
GTCACTC33700.010.3449788
ATAGGGG22000.010.33557538
GTATTAG26600.010.31951527
GGTCACT34750.010.2904967
ATTTCTA58250.010.1137322
TGGCATA22200.010.034636534
CGATAAT3155.30983E-79.9934291
CTAGCAT28250.09.95385815
GCATAGG23550.09.75050536
TAGCATC29100.09.66386216
CGACCCG29650.09.6323046
CATTTCT63400.09.3983951
CGTGGCG29750.09.3719891
Produced by FastQC (version 0.11.5)