FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004680083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004680083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44033779
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1519350.34504192792537747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC297400.027.1776074
GACCGCG298750.026.8890955
CAGACCG307100.026.4877093
ACCGCGT305000.026.4041886
TCAGACC347100.024.1225052
CCGCGTT333150.024.0245087
CGCGTTC345500.023.177198
CTCAGAC400100.022.598441
GCGTTCT381250.021.0265059
GAACGAT370200.020.92508145
TACGGAG406650.018.91138334
ATACGGA409300.018.86055233
AATACGG410200.018.85767432
AGAACGA413850.018.77239644
CAATACG427750.018.20505331
ACGGAGA431950.017.88182435
AAGAACG435800.017.8323143
CGTTCTC464550.017.75986310
CCCAATA461750.017.0936429
CGGAGAG452150.016.89875236
Produced by FastQC (version 0.11.5)