FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004680123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004680123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16434210
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG12150.026.1019256
GACCGCG39050.024.594575
ACCGCGT40300.023.8315646
AGACCGC43450.021.8969824
CGGTATA19200.021.56646320
TCGACGA15250.021.385927
CAGACCG47650.020.8170223
CCGCGTT47200.020.3477157
CGCGTTC47600.019.5624458
GTTCGAC16650.019.1825585
CGACGAG17000.018.5228968
TCAGACC58900.017.1847342
CTCAGAC83500.014.432791
GCGTTCT65750.014.3675689
CCCGTAT9100.014.1096551
ATGACGG28600.013.99857116
CTCCGTT20400.013.8026611
GTATAGA32450.013.59640322
GTCGGTT15250.013.5894211
AGTTCGA23900.013.4577314
Produced by FastQC (version 0.11.5)