FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004682244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004682244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46636128
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAT208600.012.6241111
AACTCTA115200.012.01834545
AGCTTAG134300.011.85128445
ATAGGGG142800.011.67658138
GGCATAG138800.011.60367335
CTATCGT40350.010.933874
TATATAC59550.010.6936227
GTCACTC197000.010.6000398
TCAGACG95650.010.5655582
TCTATCG46100.010.5462353
TTCTATA362050.010.44411852
TCTATAG350600.010.3875043
CACCGAA373600.010.27399945
CATAGGG161050.010.19817837
GGGAGGT235200.010.1919413
CGTGGCG161800.010.113121
GAGCTTA161900.010.09499744
GGAGGTC224150.010.0822254
GCACCGA380750.010.04546244
GCATAGG167350.09.86733936
Produced by FastQC (version 0.11.5)